Alterations of myosin isoenzymes and ADP/ATP carrier in Goldblatt hypertensive rats (part 10)

Goldblatt hypertensive rats (part 10)

Using Western immunoblot analysis, the authors have repeatedly observed marked changes in AAC protein content in wild-type cells grown under different conditions. Low levels of AAC protein were detected in cells cultured in growth media with reduced oxygen concentrations. The relative levels of AAC transcripts in wild-type cells grown in different substrates resembled the content of AAC protein in whole cells, being markedly reduced under low oxygen and low heme conditions demonstrated by Northern blot hybridization. However, in pressure-overload cardiac hypertrophy, it is possible that the relative or absolute deficiency of oxygen supply resulted in marked reduction in AAC.Serum creatine kinase and aldolase are important markers for muscle membrane damage . Because there was no significant difference in serum creatine kinase and aldolase between Goldblatt and control rats, our results also suggest that the membrane proteins, including mitochondrial membrane, may not have been damaged. Get most advantageous deals offered to you by the pharmacy you are going to appreciate soon after you become its customer: you now can get your buy generic Tavist online any time of the day or night with very fast delivery and quality guarantees.

CONCLUSIONS
Our results support the hypothesis that the shift in the myosin isoenzyme pattern towards V3 in the hypertrophied heart due to pressure overload is an adaptation that contributes to maintaining efficient force development with low oxygen and energy utilization in Goldblatt hypertensive rats. We propose that the relative or absolute deficiency of oxygen supply may have resulted in the reduction in AAC; the reduction in mitochondrial AAC may also be involved in the genesis of alterations of ventricular myosin isoenzymes in Goldblatt hypertensive rats.

This entry was posted in Hypertrophy and tagged ADP/ATP carrier, Goldblatt hypertensive rat, Hypertrophy, Myosin isoenzyme, Pyrophosphate gel electrophoresis.