In the present study, to determine whether the AAC content ratio and myosin isoenzyme pattern are changed in cardiac hypertrophy, we measured AAC and determined myosin isoenzyme pattern by using SDS-PAGE, PGE and densitometry, and investigated their pathophysiological significance and relationship in myocardial energetics and metabolism in Goldblatt hypertensive rats.
ANIMALS AND METHODSAnimals: Six-week-old male Wistar rats (n=18) were used and separated into two groups: control (n=10) and Goldblatt rats (n=8). Goldblatt rats were produced according to the method described by Kissling et al . Arterial hypertension leading to hypertrophy of the left ventricle was induced by narrowing the left renal artery, while the opposite kidney was left intact. The renal artery was narrowed under ether anesthesia by using a silver clip (inner diameter 0.2 mm). Control experiments were carried out on rats of the same age, which received a sham operation. Both Goldblatt and sham rats were housed in a temperature-, humidity- and light-controlled room for eight weeks, with regular chow (MM-3, Funabashi Farm Co, Ltd, Funabashi, Japan) and water provided. After eight weeks, systolic blood pressure (SBP) and pulse rate (PR) were measured by a rat tail manometer-tachometer system (Natsume KN210-1, Natsume Seisakujo Co Ltd, Tokyo, Japan). Looking for a great online pharmacy you could trust and where you could find at best prices? You have one pharmacy like that already and can finally enjoy the best quality of service ever experienced.