Category Archives: Adult Rat Testis

Local Regulation of Macrophage Subsets in the Adult Rat Testis: DISCUSSION(8)

However, there was no evidence of continued synthesis in the cryptorchid testis, and measurement of total testosterone in testicular homogenates collected immediately postmortem also confirmed that intratesticular testosterone levels in the cryptorchid rat were as high as, if not higher than, those in normal testes. Comparison of the IF testosterone levels and the total testicular testosterone values also indicated that testosterone is concentrated within the … Continue reading

Local Regulation of Macrophage Subsets in the Adult Rat Testis: DISCUSSION(7)

In the present study, s.c. T-implants partially reversed the increase in IF volume that follows the loss of normal vascular regulatory influences from the seminiferous tubules in the cryptorchid testis. These data suggest that the accumulation of IF following seminiferous tubule damage is partially dependent upon gonadotropin action. This effect is most likely mediated by LH, which stimulates IF volume in other physiological and pathophysiological … Continue reading

Local Regulation of Macrophage Subsets in the Adult Rat Testis: DISCUSSION(6)

In spite of the considerable experimental evidence that Leydig cells maintain the elevated number of macrophages in the testis, the mechanism involved remains to be established. It may involve direct contact via Leydig cell-mac-rophage specializations, although the relatively slow decline in macrophage numbers following Leydig cell destruction by EDS treatment suggests that simple physical attachment is not the mechanism responsible. Other cytokines may be involved; … Continue reading

Local Regulation of Macrophage Subsets in the Adult Rat Testis: DISCUSSION(5)

Consistent with our previous data that testicular MIF mRNA and protein levels are maintained following hy-pophysectomy, MIF was only slightly affected by the changes in LH, FSH, and testosterone induced by T-im-plants in the present study. The results did indicate that MIF levels decline in the abdominal testis, but there was no apparent positive relationship between the levels of this cytokine in testicular IF and … Continue reading

Local Regulation of Macrophage Subsets in the Adult Rat Testis: DISCUSSION(4)

In contrast, the number of monocyte/macrophages (ED2~ cells) was regulated by the changes in testosterone in the scrotal testis. Although there have been reports of direct actions of testosterone on macrophages, and monocytes in particular, the observation that the testosterone responsiveness of the ED2~ population was lost in the abdominal testis indicates that this particular action may be mediated via the seminiferous tubules. In the … Continue reading

Local Regulation of Macrophage Subsets in the Adult Rat Testis: DISCUSSION(3)

The failure of experimental bilateral cryptorchidism to alter macrophage number in the adult rat is entirely consistent with previous studies that have reported no change in total macrophage absolute volume in abdominal rat and mouse testes using a similar experimental model, indicating that neither testicular macrophage number nor size is affected by disruption of seminiferous tubule function. Although total macrophage volume has been shown to … Continue reading

Local Regulation of Macrophage Subsets in the Adult Rat Testis: DISCUSSION(2)

Both nuclear diameter and total macrophage numbers were slightly greater in this study compared with a previous study using identical techniques. This may be attributed at least partially to the fact that the average testis weight also was slightly larger in the present study, because total macrophage number was found to be correlated with organ weight, and hence interstitial tissue volume, in the normal untreated … Continue reading

Local Regulation of Macrophage Subsets in the Adult Rat Testis: DISCUSSION(1)

In adult rats, reducing Leydig cell function by inhibition of gonadotropin secretion with T-implants led to a decrease in resident testicular macrophages 3 wk later, similar to the decline observed at the same time point after ablation of the Leydig cells with EDS, providing further confirmation of the importance of functional Leydig cells for maintaining normal resident testicular macrophage numbers. The decline in macrophage numbers … Continue reading

Local Regulation of Macrophage Subsets in the Adult Rat Testis: RESULTS(6)

The number of cells in the ED1+ED2~ monocyte/macrophage subset was not clearly correlated with any of the above parameters but did show a strong correlation with the number of ED2+ED1~ (long-term resident) macrophages (Fig. 7C). There also was a significant relationship between IF volume and the number of these long-term resident macrophages (Fig. 7D), but not between IF volume and the other macrophage parameters. buy asthma inhaler

Local Regulation of Macrophage Subsets in the Adult Rat Testis: RESULTS(5)

In contrast to observations in the scrotal testis, the number of ED1+ED2~ monocyte/macrophages was not significantly different from normal control levels in any abdominal testis regardless of the presence or absence of either length of T-implant (Fig. 6C). However, the T24-implants did partially restore ED2+ resident macrophage numbers in the abdominal testis (Fig. 6B) without any apparent effect on the number of ED1+ED2~ monocyte/macrophages (Fig. … Continue reading