Category Archives: Bordetella pertussis

An improved immunofluorescent reagent for detection of Bordetella pertussis: part 5

Flow cytometry can be easily adapted for the accurate detection of B pertussis cells directly in clinical specimens. Flow cytometers are designed to count particles efficiently by size and protein content using light scattering. In our case, the instrument was adjusted by preliminary experiments to detect particles matching B pertussis cell size and nucleic acid content. To increase the specificity of the test, the fluorescein-labelled … Continue reading

An improved immunofluorescent reagent for detection of Bordetella pertussis: part 4

It is important to note that only five of the 1398 specimens tested gave positive results while the culiure remained negaitive. To verify whether B pertussis cells are present or absent on these slides, each sample is eluted and then tested by pcr. Recently, it was observed that patients for whom diagnosis of infectious diseases was made more rapidly had a significantly lower mortality rate. … Continue reading

An improved immunofluorescent reagent for detection of Bordetella pertussis: part 3

The unique antigenic site recognized by the MAbs is located on three amino sugars of the carbohydrate portion of B pertussis los. This reactive site is antigenically very stable and highly conserved among all B pertussis isolates regardless of physiological state. Only very rare laboratory los variant strains do not have these three amino sugars on the los molecule and are therefore not recognized by … Continue reading

An improved immunofluorescent reagent for detection of Bordetella pertussis: part 2

Isolation of B pertussis from nasopharyngeal swabs or aspirations remains the ‘gold standard’ for the diagnosis of pertussis infection. This method is highly specific but is time-consuming, labour intensive and results are obtained only after four to seven days of culture on specialized media. Culture is available in many clinical laboratories but has a low sensitivity of 40 to 60%. Serological analysis by a variety … Continue reading

An improved immunofluorescent reagent for detection of Bordetella pertussis: part 1

INFECTION WITH BORDETELLA PERTUSSIS IS STILL AN IMPORTANT ┬ápublic health problem, and outbreaks of pertussis continue to occur in Canada. In 1992, over 3700 cases of pertussis infection were reported in Canada . Moreover, 7049 and 3218 cases of pertussis were reported for 1993 and the first half of 1994, respectively, indicating a rise in the incidence of this disease in Canada (personal communication). The … Continue reading