Category Archives: Cigarette Smoke - Part 2

Identification of Cigarette Smoke Components: MATERIALS AND METHODS(6)

Phenol Phenol was assayed using a method based upon the in-dophenol reaction. To 1 ml of standards (3-9 |a.M phenol) and cigarette smoke samples in 25-cm glass tubes was added 500 jjlI 0.01% ammonium chloride. After mixing, 1 ml of 0.03% sodium nitroprusside was added to each tube and mixed. One milliliter of 7V-chlorosuccinimide-succi-nimide reagent (made by dissolving 25 mg of succinimide and 60 mg … Continue reading

Identification of Cigarette Smoke Components: MATERIALS AND METHODS(5)

Formaldehyde The formaldehyde content of cigarette smoke solutions was determined by using a chromotropic acid method. To 1 ml of cigarette smoke samples and standards (1-7 |xg/ml formaldehyde) in 25-cm glass test tubes, 25 (jlI of 1% chromotropic acid was added and mixed. Six milliliters of concentrated sulfuric acid was then added to each tube. The tubes were cooled to room temperature, and the absorbance … Continue reading

Identification of Cigarette Smoke Components: MATERIALS AND METHODS(4)

Assay of Cigarette Smoke Solution Components The cigarette smoke solutions were assayed for the concentration of five compounds reported to be present in cigarette smoke and known to inhibit cilia in other organs. Spectrophotometric methods were chosen that could assay these compounds in complex smoke solutions reliably without interference from other components. Appropriate dilutions of freshly made smoke solutions were made into the assay medium … Continue reading

Identification of Cigarette Smoke Components: MATERIALS AND METHODS(3)

MS gas-phase cigarette solutions were prepared in the same manner as whole MS solutions except that a Cambridge filter (University of Kentucky, Lexington, KY) was interposed between the puffer box and the collection flask. This filter was replaced with a fresh filter after 30 puffs had been generated. To prepare the MS particulate-phase cigarette solutions, each filter used during collection of the MS gas phase … Continue reading

Identification of Cigarette Smoke Components: MATERIALS AND METHODS(2)

Solutions of cigarette smoke generated for the assay of cyanide were collected in EBSS-H medium. For the assay of phenol, methanol was added to 1%, and the EBSS-H collection media for acrolein and formaldehyde contained 1% NaHS03. All chemicals were reagent grade. The chemicals used to make EBSS-H and to assay concentrations of acrolein, formaldehyde, phenol, and potassium cyanide (KCN) in smoke solutions were purchased … Continue reading

Identification of Cigarette Smoke Components: MATERIALS AND METHODS(1)

Animals Female golden hamsters (Mesocricetus auratus) purchased from Harlan Sprague Dawley (San Diego, CA) at 9-12 wk of age were maintained on a 14L:10D cycle in a room at 26°C. No more than four females were grouped in a cage. Purina rodent chow and water were provided ad libitum. Females were evaluated in the morning for the presence of a vaginal discharge (Day 1 of … Continue reading

Identification of Cigarette Smoke Components That Alter Functioning of Hamster(2)

Cigarette smoke is a complex mixture of over 4000 chemicals, and cigarette smoke solutions made in physiological media likewise contain numerous different types of chemicals that include the more water-soluble components of cigarette smoke. Cigarette smoke is toxic to cilia of other organs, including the mammalian respiratory tract, and to cilia of model systems such as Paramecium and mussel gill. Various chemicals in cigarette smoke, … Continue reading

Identification of Cigarette Smoke Components That Alter Functioning of Hamster(1)

The oviduct plays two critical roles in mammalian reproduction. It functions in pick-up and transport of the gametes, and it provides a suitable milieu for fertilization and preimplantation development. In hamsters, the outer surface of the infundibulum is covered mainly by ciliated cells. After ovulation, these ciliated cells pick up the oocyte cumulus complex (OCC) and transfer it to the ampulla, where fertilization occurs. buy ortho tri-cyclen online