Category Archives: Pulmonary Fibrosis

Oligo-2,5-Adenylate Synthetase in Pulmonary Sarcoidosis and Idiopathic Pulmonary Fibrosis: Conclusion

Against our expectation, in NC the levels of BALF cell 2,5AS activity were prominently high as compared with the levels of PBML 2,5AS activity (approximately sevenfold of the PBML activity). To clarify whether the levels of 2,5AS activity depend on cell differentiation, we analyzed the correlations of the 2,5AS activity levels to ratios of cells composed in PBML and BALF cells, and the levels of … Continue reading

Oligo-2,5-Adenylate Synthetase in Pulmonary Sarcoidosis and Idiopathic Pulmonary Fibrosis: Discussion

In PBML of patients with SAR and IPF, the correlations between the levels of 2,5AS activity and the ratios of monocytes to lymphocytes were analyzed. In PBML of patients with IPF and SAR, there were no significant correlations between them (data not shown). No significant correlation between the levels of BALF cell 2,5AS activity and the ratios of lung lymphocytes to PAM was found in … Continue reading

Oligo-2,5-Adenylate Synthetase in Pulmonary Sarcoidosis and Idiopathic Pulmonary Fibrosis: Results

Characteristics of BALF Cells Table 1 shows the data on recovery’, total cell, and differential cell counts in BALF cells. The recovery rates of SAR, IPF, and NC were 65.3 ± 4.8 percent, 52.3 ± 9.4 percent, and 66.9 ± 3.8 percent, respectively. The recovery rate of IPF was significantly decreased as compared with that of NC. The total cell number per milliliter of BALF … Continue reading

Oligo-2,5-Adenylate Synthetase in Pulmonary Sarcoidosis and Idiopathic Pulmonary Fibrosis: Methods

Bronchoalveolar Lavage Three 50-ml aliquots of 0.9 percent sterile saline solution were instilled into a bronchus in the left lingular division or the right middle lobe through a fiberoptic bronchoscope. The BALF was recovered by gentle suction immediately after the infusion of each aliquot. After centrifugation, the recovered cells were washed three times with phosphate-buffered saline solution (PBS). A small volume was removed for cell … Continue reading

Oligo-2,5-Adenylate Synthetase in Pulmonary Sarcoidosis and Idiopathic Pulmonary Fibrosis

Treatment of cells with all types of interferon (IFN) the induction of oligo-2′,5′-adenylate synthetase (2,5AS) which is responsible for the synthesis of 2′-5′-linked oligomers of adenosine (2,5a). The enzyme product 2,5A itself has the ability to inhibit protein synthesis and to regulate cell growth. In this regard, 2,5AS plays a very important role in the mechanism of the action of IFN. This enzyme activity thus … Continue reading