Changes in Uterine Expression of Leukemia: MATERIALS AND METHODS(1)

MATERIALS AND METHODS(1)Reagents

Ketamine hydrochloride was obtained from Fort Dodge Laboratories (Fort Dodge, IA). Sodium pentobarbital was obtained from Abbott Laboratories (North Chicago, IL). Penicillin G was obtained from Solvay Animal Health, Inc. (Mendota Heights, MN). Recombinant human LIF (rhLIF) and anti-hLIF neutralizing antibody were obtained from R&D Systems, Inc. (Minneapolis, MN). Guanidine thio-cyanate, guanidine hydrochloride, buffer-saturated phenol, and cesium chloride were obtained from Amresco (Dallas, TX). Tris-HCl, Tris base, sodium citrate, and sodium hydroxide were purchased from Fisher Scientific (Pittsburgh, PA). antibiotics levaquin

Specific DNA primers, Taq polymerase, Superscript II, and restriction enzymes were obtained from Gibco BRL (Gaithersburg, MD). RQ1 DNase, recombinant RNasin, and random hexamers were obtained from Promega (Madison, WI). AmpliTaq Gold polymerase was obtained from Per-kin-Elmer (Branchburg, Nj). All radioactive isotopes were obtained from NEN-DuPont (Boston, MA). Normal rabbit serum and goat IgG were obtained from Vector Laboratories (Burlingame, CA). Nytran Plus membranes were purchased from Schleicher & Schuell (Keene, NH). All other chemicals were purchased from Sigma Chemical Co. (St. Louis, MO).

This entry was posted in Leukemia and tagged Inhibitory Factor, Leukemia, Pregnancy.