Determination of Plasma Membrane Characteristics: RESULTS(2)

Cryoprotectant permeability (PCpa) was determined to be 0.316 ± 0.290, and 0.279 ± 0.280 X 103 cm/min for glycerol, at 8°C and 0°C, respectively, and 1.08 ± 0.068 and 0.704 ± 0.033 X 10“3 cm/min for EG, at 8°C and 0°C, respectively. Reflection coefficients were determined to be 0.852 ± 0.028, and 0.812 ± 0.026, for glycerol and water at 8°C and 0°C, respectively. Reflection coefficients were determined to be 0.719 ± 0.017 and 0.561 ± 0.041 for EG and water at 8°C and 0°C, respectively. The activation energies (Ea) for Lp in the presence of glycerol and EG were estimated, using the Arrhenius relationship, to be 7.80 and 11.51 Kcal/mol, respectively. The estimated activation energies for Pcpa were 4.06 and 7.48 Kcal/mol for glycerol and EG, respectively, and can be seen in Figure 3. buy prednisone

Fig3Determination of plasma membrane
FIG. 3. An Arrhenius plot of the Lp (circles) and PEC (A) or Р6,усегЫ (squares) (B) of boar spermatozoa. C) Same as A but in the presence of Modena extender. Permeability values were determined at22°C, 8°C, and 0°C (n = 3 at each temperature).

Experiment 3: Osmotic Tolerance Limits of Boar Spermatozoa in the Presence of Extender

Figure 2 shows the relative percentage of motile cells as a function of osmolality. Motility was evaluated in anisosmotic conditions, and 1 and 5 min after return to isosmo-lality. Results indicate that there was no significant effect of treatment (p = 0.849) on motility. However, the results did indicate that there is a significant interaction (p = 0.0001) between extender and osmolality. Table 3 summarizes the percentages of volume excursions cells can tolerate during swelling and shrinking while still maintaining 90%, 80%, and 70% motility, respectively.

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