Development and Validation of a Fecal Testosterone Biomarker: DISCUSSION(1)

DISCUSSION(1)

The use of feces for steroid analysis enables laboratory and field data to be collected in situations in which blood and urine collection may prove either detrimental to the animal or impossible to obtain. Blood collection is an invasive technique through which only limited number of samples can be obtained from a single animal. Furthermore, fecal measurements of testosterone correlate well with measurements of plasma testosterone. The method described and evaluated in this paper combines a noninvasive and simple collection procedure with an analytical technique that has been shown to accurately measure testosterone concentration. The methods were designed for eventual use of the technique as a biomarker to be used on field populations. buy ventolin inhalers

The specificity of the assay gives good evidence of a highly specific assay. The only compound that was found to cross-react with the antibody at > 1% was 5a-DHT. This does not present a problem because 5a-DHT is the more potent form of testosterone, is found in much lower concentrations, and is also of biological interest because of the equivalent cellular targets of both compounds.

This entry was posted in Testosterone and tagged Biomarker, Mus musculus, Peromyscus maniculatus, Testosterone.