The goals of this study were to determine which components) in cigarette smoke solutions inhibits oviductal CBF and whether inhibition of beat frequency correlates with a reduction in OPR. All of the smoke solution components that were tested inhibited CBF in hamster oviductal explants in a dose-dependent manner. Their hierarchy of potency and their inhibitory concentrations for CBF were as follows: KCN (50 |xM) > formaldehyde (1.66 mM) > acrolein (1.78 mM) > phenol (10 mM). This hierarchy is in good agreement with data obtained using cilia from the trachea or from model systems such as mussel gill cilia. In our hierarchy, KCN was over 33 times more potent an inhibitor of CBF than formaldehyde, which was second in potency. buy ortho tri-cyclen
The concentrations of acrolein, formaldehyde, and phenol required to inhibit CBF during acute exposure were higher than the concentrations of these components in any of the six types of smoke solutions studied. In contrast, KCN inhibited both CBF and OPR at concentrations found in each type of smoke solution. Thus cyanide accounts for at least part of the inhibition of CBF and OPR observed when smoke solutions are applied to infundibular explants. Cyanide concentrations were highest in MS whole and MS gas-phase smoke solutions, which were both found previously to be more potent inhibitors of CBF than MS particulate or any type of SS smoke. Our study also shows that both CBF and OPR are highly sensitive to cyanide. The cyanide concentrations that inhibit CBF (50 |xM) and OPR (15 |xM) are 200 and 666 times lower, respectively, than the concentration (10 mM) normally used to inhibit ATP production.