Solutions of cigarette smoke generated for the assay of cyanide were collected in EBSS-H medium. For the assay of phenol, methanol was added to 1%, and the EBSS-H collection media for acrolein and formaldehyde contained 1% NaHS03.
All chemicals were reagent grade. The chemicals used to make EBSS-H and to assay concentrations of acrolein, formaldehyde, phenol, and potassium cyanide (KCN) in smoke solutions were purchased from Mallinckrodt (Paris, KY), Fisher Scientific (Fairlawn, NJ), and Calbiochem (La Jolla, CA). BSA (fraction V, tissue culture certified), hCG, acrolein, and phenol were obtained from Sigma Chemical Company (St. Louis, MO). Formaldehyde was obtained from Fisher Scientific. KCN was purchased from Mallinckrodt. buy asthma inhalers
Preparation of Cigarette Smoke Solutions
Whole MS cigarette smoke solutions were prepared immediately before use by generating 2.2-sec, 40-ml puffs, once per minute, from University of Kentucky 2R1 Reference cigarettes. A puffer box and a peristaltic pump were used to draw the smoke into a 25-ml flask containing 10 ml of EBSS-H medium. After each puff, the vacuum on the line was released, and the smoke remained in contact with the solution until 10 sec before the production of another puff. The vacuum was reapplied to clear the smoke from the flask. This procedure was repeated until 60 puffs of smoke had been generated.