Immunohistochemical Distribution of Follistatin: MATERIALS AND METHODS(9)


Similarly, the gray-scale standard deviation values for the positive reaction were converted to a scale of 0-100 and were defined as heterogeneity. Intra- and interday coefficients of variation for gray-scale densitometry were 6.8% and 8.5%, respectively. Used in this fashion, the absorptive index is an indicator of the amount (concentration) of follistatin in the tissue but is independent of the pattern of distribution of follistatin within the sample. Heterogeneity indicates the relative difference in distribution at the tissue level, that is, how evenly the reaction product is distributed within the tissue. canadian health & care mall

Statistical Analysis

Data for the corpus luteum were examined by ANOVA to detect a day effect. Data for follicles were examined by 2-factor ANOVA to determine the effect of follicle type, day of wave, and follicle-by-day interaction. If the analysis resulted in a probability value of < 0.05, multiple comparisons were made using the method of least significant difference. Initially, data for absorptive index and heterogeneity were analyzed for differences among dominant, largest subordinate, and second-largest subordinate follicles (Fig. 1); however, as no statistical differences were detected between largest and second-largest subordinate follicles, the data were reanalyzed after subordinate follicles were combined into a single group, and results are presented as such.

This entry was posted in Follicles and tagged Cattle, Follicles, Follistatin, Immunohistochemical Distribution.