Both nuclear diameter and total macrophage numbers were slightly greater in this study compared with a previous study using identical techniques. This may be attributed at least partially to the fact that the average testis weight also was slightly larger in the present study, because total macrophage number was found to be correlated with organ weight, and hence interstitial tissue volume, in the normal untreated testes. It is most important to note that testicular macrophage numbers and nuclear diameter data both in the present study and in the previous study employing perfusion-fixed frozen testes are approximately the same as testicular macrophage numbers obtained using more ‘‘effective” glutaraldehyde fixation and plastic-embedded testis tissue, which is more resistant to changes in volume caused by processing and sectioning. buy asthma inhalers
Comparison of the macrophage density data and number data for normal versus cryptorchid rat testes in the present study reinforces the importance of using absolute cell numbers, rather than density, in studies of changes in cell populations in the testicular interstitial tissue, particularly when one is dealing with treatments that cause large changes in seminiferous tubule volume.