The remaining testis was fixed by lower body perfusion via the descending aorta, with 2% paraformaldehyde, 75 mM lysine, 10 mM periodate, containing 1.0% glutaralde-hyde, and then weighed and processed for collection of 6-^m frozen sections, as previously described. Animals were not pretreated with any anti-clotting agents. buy levaquin online
After collection of the testes, several androgen-responsive tissues, i.e., the thymus, seminal vesicles (including coagulating glands), and epididymis, were removed and weighed to ensure that the T-implants had been successful, and the vasa deferentia were examined macroscopically to ensure that these had not been damaged during surgical induction of cryptorchidism. This latter observation was considered necessary since vasectomy in certain rat species can cause spontaneous testicular inflammation that could compromise the study.
In order to provide additional validation of the intrates-ticular testosterone levels in cryptorchidism, testes from groups of untreated or 4-wk bilaterally cryptorchid adult rats (n = 6 animals per treatment group) were used for collection of IF as described above (left testis) or immediately snap-frozen in liquid nitrogen (right testis) prior to homogenization in 1.5 ml/g tissue PBS. An additional group of cryptorchid rats was used for collection of testicular IF without drainage (right testis) or after overnight drainage (16 h, 4°C, left testis). The IF and testicular ho-mogenate samples were stored at – 20°C prior to assay.