Local Regulation of Macrophage Subsets in the Adult Rat Testis: RESULTS(2)

Hormonal and Accessory Organ Observations

Serum LH and FSH levels were increased 2-fold in cryptorchid rats (Fig. 2, A and B). Serum LH was reduced to 30% of normal control levels by either length of T-im-plant in rats with either scrotal or abdominal testes (Fig. 2A), while serum FSH was reduced to a lesser degree (5080% of normal) under the same conditions (Fig. 2B).

Testicular IF testosterone levels measured without extraction were significantly higher in the abdominal testis compared with levels in normal controls (Fig. 3A). The T3-implants reduced IF testosterone in both scrotal and abdominal testes to less than 5% of normal, while T24 maintained IF testosterone levels at about 10% of the control value, which was sufficient to maintain testis weights and spermatogenesis. By contrast, seminal vesicle weights were unaffected by cryptorchidism, were slightly stimulated (3040%) by T3-implants, and were increased 2-fold by T24-implants in rats with either scrotal or abdominal testes (Fig. 3B). Thymus weights were reduced by 50% in the T24-implanted rats but were unaffected by either cryptorchidism or T3-implants (Fig. 3C). These data confirmed that the T3-implants successfully maintained circulating testosterone at close to normal levels, while the T24-implants produced superphysiological levels of testosterone in the serum. ventolin inhaler
Fig2Local Regulation
FIG. 2. Serum LH (A) and FSH (B) levels in adult rats with scrotal or abdominal testes without implants (C, Co), with T3-implants, or with T24-implants. Values with same letter subscript are not significantly different (p > 0.05).

Fig3Local Regulation
FIG. 3. IF testosterone levels and androgen-dependent organ weights for adult rats with scrotal or abdominal testes without implants (C, Co), with T3-implants, or with T24-implants. A) IF testosterone (testo). Note log scale on y-axis. B) Seminal vesicle weights. C) Thymus weights. Values with same letter subscript are not significantly different (p > 0.05).

This entry was posted in Adult Rat Testis and tagged Adult Rat Testis, Inhibitory Factor, Macrophage, Seminiferous Tubules, Testosterone.