Local Regulation of Macrophage Subsets in the Adult Rat Testis: RESULTS(3)

RESULTS(3)

Testicular MIF Concentrations

Immunoreactive MIF levels in testicular IF measured by ELISA (Fig. 4) and by Western blot analysis (Fig. 5) were unaffected in all groups with scrotal testes but were reduced in the abdominal testis. Both T3- and T24-implants at least partially restored immunoreactive MIF levels in the abdominal testes. Accurate quantification of MIF protein levels from the Western data was not considered because of overloading of the gel, which was clearly apparent from the retention of protein between the running lanes at the loading gel/resolving gel interface, and also because the loaded samples were pools of the residual IF available after the testosterone RIA and MIF ELISA. Buy Asthma Inhalers Online

Macrophage Quantitation

The number per unit testis volume of total monocyte/ macrophages, identified by combined immunohistochemi-cal localization of ED1 and ED2, was increased 3-fold following cryptorchidism (Table 1). There was a clear difference between the maximum nuclear diameters of the ED1 + and ED2+ cells (5.7 ± 0.1 and 6.7 ± 0.1 ^m, respectively, in scrotal testes), and cryptorchidism had no effect on these parameters (5.9 ± 0.1 and 6.8 ± 0.3 ^m, respectively, in abdominal testes).
Fig4Local Regulation
FIG. 4. MIF immunoreactivity measured by ELISA in testicular IF from adult rats with scrotal or abdominal testes without implants (C, Co), with T3-implants, or with T24-implants. Values are mean ± SEM (number of animals in parentheses above histograms). Values with same letter subscript are not significantly different (p > 0.05).

Fig5Local Regulation
FIG. 5. MIF immunoreactivity detected by Western blot analysis in pooled samples of testicular IF from adult rats with scrotal or abdominal testes without implants (C, Co), with T3-implants, or with T24-implants. Arrow: loading gel/resolving gel interface; muMIF: recombinant murine MIF standard.

TABLE 1. Numerical density estimates for total testicular macrophages in scrotal and abdominal testes.*

Experimental group T-implant length Macrophage density (cells/cm3 X 10-6)
Scrotal testes 0 7.50 ± 0.65a
3 cm 4.96 ± 0.17b
24 cm 4.88 ± 0.25b
Abdominal testes 0 21.9 ± 2.2c
3 cm 16.4 ± 1.5d
24 cm 18.3 ± 2.3cd

* Values are mean ± SEM (n = 6 animals); values with same letter superscript are not significantly different (p > 0.05).

This entry was posted in Adult Rat Testis and tagged Adult Rat Testis, Inhibitory Factor, Macrophage, Seminiferous Tubules, Testosterone.