Total testicular macrophage numbers (i.e., all ED1+ cells and ED2+ cells) declined by approximately 40-50% in T3-implanted and in T24-implanted rat testes (Fig. 6A). This decline was primarily the result of a 40% reduction in the number of resident macrophages (ED2+ cells only) (Fig. 6B). There was a more pronounced decline (75%) in the less numerous ED1+ED2~ monocyte/macrophage subset in the T3-implanted animals, while the numbers of this subset were maintained in the T24-implanted group (Fig. 6C). The ED2+ED1~ (long-term resident) macrophages comprised between 25-43% of the ED2+ macrophage population across all treatment groups, and no significant difference in the number of ED2+ED1~ between the treatment groups was detected (data not shown). buy ortho tri-cyclen
In spite of the large increase in macrophage density observed in the abdominal testis (Table 1), there was no change in the total number of intratesticular monocyte/macrophage or resident macrophages (Fig. 6, A and B). This apparent discrepancy between cell density and number was due to the very large decrease in testis volume following cryptorchidism.
FIG. 6. Total testicular macrophages (A), ED2+ resident testicular macrophages (B), and ED1+ED2- testicular monocyte/macrophages (C) in adult rats with scrotal or abdominal testes without implants (C, Co), with T3-implants, or with T24-implants. Values with same letter subscript are not significantly different (p > 0.05).