Role of nitric oxide synthase types II and III in early protection against endotoxin-induced lung injury: ANIMALS AND METHODS (part 2)

Survival analysis: Survival curves were plotted in terms of probability of survival (y axis) as a function of survival time (x axis) by using the Kaplan-Meier method . Survival curves were compared by the log-rank test using GraphPad Prism statistical package (GraphPad Software, San Diego, California, USA). Animal models and tissue samples: Twelve-week-old male Sprague-Dawley rats were used. Control (untreated) and LPS-treated rats were killed by decapitation 15,25,30,60 and 120 mins after LPS infusion. Freshly excised lung, kidney, heart, spleen and liver were either fixed for in situ hybridization and immunochemistry as previously described for pathological examination or immediately submitted to RNA isolation.
Preparation of specific digoxigenine-labelled cDNA probes: Double-strand cDNA probes specific for NOS-II and NOS-III mRNA were amplified and labelled using PCR technology . Briefly, two-stage PCR procedures were performed; in primary PCR, a sufficient amount (10 to 50 ng) of unlabelled template DNA was produced. Isolated plasmid DNA containing a complete (4004 base pairs) insert for NOS-II or partial (693 base pairs) rat NOS-III sequence was used as a template (1 to 10 pg) . You can get all the drugs you want for treating your problem in no time if you order it at the . We are recommending this pharmacy with confidence because it already has thousands of satisfied customers.

This entry was posted in Lung injury and tagged 1-Amino-2-hydroxy-guanidine, Endotoxic shock, NG-nitro-L-arginine, Nitric oxide, Nitric oxide synthase type II, Nitric oxide synthase type III, S-nitroso-N-acetyl-penicillamine.