Role of nitric oxide synthase types II and III in early protection against endotoxin-induced lung injury: RESULT (part 3)

A single 324 base pair product for NOS-III was amplified (lane 2). Primers specific for beta-actin were also used, and results were compared (band 308 base pairs, lane 3). No bands were detected when primers specific for NOS-II or no primers (negative control) were used. In the second series of experiments, rats were decapitated 30 mins after LPS infusion, and freshly isolated RNA was analyzed by RT-PCR. The 324 base pair product specific for NOS-III (lane 5) and the 384 base pair product specific for NOS-II (lane 4) were detected (Figure 4). flovent inhaler

 

Role of nitric oxide synthase types II and III in early protection against endotoxin-induced lung injury

Figure 4 Recombinant Thermus thermophilus reverse transcription polymerase chain reaction (RT-PCR) amplification of inducible nitric oxide synthase (NOS-II) and constitutive NOS (NOS-III) mRNA in rat lung 30mins after lipopolysaccharide (LPS) infusion. Freshly isolated total RNA from rat lung was reverse transcribed and amplified with rat gene-specific primers for NOS-II or NOS-III isoforms, and analyzed by agarose gel electrophoresis, with ethidium bromide staining. A 324 base pair (bp) band of NOS-III transcript (lane 5) and a 384 bp PCR product of beta-actin were observed (positive control). No signal was obtained in the absence of any primers (negative control, lane 3). Marker M1 is shown in lane 1 (pUC19/MspI with bands 501/489, 404, 331, 242, 190, 147 bp long). The size of the predicted amplified products is indicated on the right

This entry was posted in Lung injury and tagged 1-Amino-2-hydroxy-guanidine, Endotoxic shock, NG-nitro-L-arginine, Nitric oxide, Nitric oxide synthase type II, Nitric oxide synthase type III, S-nitroso-N-acetyl-penicillamine.