Role of nitric oxide synthase types II and III in early protection against endotoxin-induced lung injury: RESULT (part 7)

RESULT (part 7)

NOS-II mRNA was detected in all tested organs of LPS-treated rats after 2 h. The NOS-II transcripts appeared as early as 30 mins after LPS infusion in the lung and kidney (Figure 8). To ensure that it was possible to detect NOS-II transcript in lung tissue treated for 30 mins with LPS, Northern blot analysis was performed using a DIG-labelled 473 base pair cDNA probe.  To identify the lung cell type expressing the NOS-II mRNA, in situ hy-bridization with specific 473 base pair DIG-labelled cDNA probe was performed on sections of rat lung. buy antibiotics online

Role of nitric oxide synthase types II and III in early protection against endotoxin-induced lung injury

Figure 8 Colorimetric dot blot showing inducible nitric oxide synthase (NOS-II) mRNA transcript. Total RNA was isolated from control and lipopolysaccharide- (LPS) treated rats decapitated 15, 30, 60 and 120 mins after LPS infusion. RNA from lung, liver, spleen, heart and kidney was blotted onto a nylon membrane and hybridized with digoxigenine- (DIG) labelled NOS-II-specific 473 base pair cDNA probe. DIG-labelled cDNA probe for ribosomal 18S RNA was used as a positive control. Hybridization and detection were performed as described in Animals and Methods. No NOS-IImRNA was detected in control rat tissues or in rat tissues infused for 15 mins with LPS, but a strong signal was detected 30 mins after LPS infusion in samples from lung and kidney. Two hours after LPS infusion, a specific NOS-II mRNA signal was present in the heart, spleen and liver

This entry was posted in Lung injury and tagged 1-Amino-2-hydroxy-guanidine, Endotoxic shock, NG-nitro-L-arginine, Nitric oxide, Nitric oxide synthase type II, Nitric oxide synthase type III, S-nitroso-N-acetyl-penicillamine.